Title: Effects of glyoxal on anti-oxidative system in mice
Abstract: objective:To study the effects of glyoxal on anti-oxidative system in mice and its mechanism of toxicity through measuring the content of SOD(Superoxide Dismutase)、GSH-Px(Glutathione Peroxidase)、T-SH(Total Sulfhydryl)、NP-SH(Non-Protein Sulfhydryl)and LPO(Lipid Peroxidation)in the blood and tissue in mice treated with glyoxal.Method:This study use 40 mice of KunMing species,which are divided into 4 groups evenly on random.1 is negative control group treated with normal saline,other 3 is experiment groups treated with glyoxal of 1.3mmol/kg(1/8LD50)、2.6mmol/kg(1/4LD50)and 5.2mmol/kg(1/2LD50)separately.The mice were taken blood、heart、liver、brain and kidney after they were treated with glyoxal for 24 hours.The activity of GSH-Px in blood and tissue were measured by DTNB method;The content of T-SH and NP-SH in blood and tissue were measured by Ellmans,and the content of PB-SH can be got from T-SH subtracting NPSH.The activity of SOD in blood and tissue were measured by nitrite method.The content of LPO(especially MDA)in serum and tissue was measured by TBA method.Results:The content of LPO in various dose groups in brain and in high dose group in liver of mice is significantly higher than that of the control group(P0.05 or P0.01).The activity of SOD in liver and brain of high dose groups increases.The activities of GSH-Px significantly decrease in blood and kidney at any dose groups,in liver high and middle dose groups decrease.The content of T-SH and PB-SH increases in blood and brain of high dose groups.The content of NP-SH decreases in brain and kidney of high dose groups and in liver of middle and low dose groups.Comparing with control group,the difference is notable(P0.05 or P0.01).Conclusion:The liver and brain of mice have high sensitivity to free radical caused by Glyoxal,and they were damage easily by Lipid Peroxidation;The activity of GSH-Px in blood 、liver and kidney is the sensitive index expose to glyoxal.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot