Title: Determination of Naringin,Hesperidin and Neohesperidin in Tongfu Liqi Granules by HPLC
Abstract: Objective To explore a method for simultaneously determining naringin,hesperidin and neohesperidin in Tongfu Liqi Granules.Methods HPLC method was used.Kromasil C18(250 mm×4.6 mm,5 μm) column was adopted.The mobile phase was composed of acetonitrile(A) and water(B).The detection wavelength was 283 nm.The flow rate was 1.0 mL/min and the column temperature was 30 ℃.Results The calibration curve was linear within the range of 10.4-166.2,6.1-98,21.2-339.2 μg for narigin,hesperidin and neohesperidin,respectively.The average recovery was 98.14%,98.36% and 96.94% for narigin,hesperidine and neohesperidine,respectively,and RSD was 1.58%,1.79% and 2.75%.Conclusion The method is reproducible,sensitive and accurate for determination of narigin,hesperidin and neohesperidin in Tongfu Liqi Granules.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot