Title: Contents determination of Naringin,hesperidin and neohesperidin in processed products of Fructus Aurantii by HPLC
Abstract: Objective: To determine the contents of Naringin,hesperidin and neohesperidin in Fructus Aurantii and its products processed by Jiangxi methods and pharmacopoeia metheds by HPLC.Methods: E1823028 C18 Column(250mm×4.6mm,5μm),mobile phase of acetonitrile-0.1%phosphoric acid(volume ratio of 23:77),detection wavelength at 285nm.flow phase rate being 1.0mL/min and the column temperature at 30℃.Results: The calibration curves were linear in the range of 0.596-5.96μg for narngin(r=0.9997),0.106-1.06μg for hesperidin(r=0.9996)and 0.470-4.70μg for neohesperidin(r=0.9999).The average recovery of naringin was 101.17%,(RSD=1.26%),the average recovery of hesperindin was 101.2%(RSD=0.895%) and that of neohesperindin was 101.28%(RSD=1.34%).Conclusion: This method is simple,accurate and reliable for the quality control of Fructus Aurantii.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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