Abstract: AIM To establish the quality standard for Dachaihu Granules(Bupleuri Radix,Scutellariae Radix,Rhei Radix et Rhizoma,Aurantii Fructus immaturus and Paeoniae Radix alba,etc.).METHODS TLC was used for the qualitative identification of Scutellariae Radix,Rhei Radix et Rhizoma,Aurantii Fructus immaturus and Paeoniae Radix alba.HPLC was used to simultaneously determine the contents of naringin,hesperidin,neohesperidin and baicalin.The samples were separated on a Kromasil C18(250 mm×4.6 mm,5 μm) column by a gradient elution using acetonitrile-0.2% acetic acid as the mobile phase and detection wavelength was set at 280 nm.RESULTS TLC spots were clear and well-separated without negative interference.HPLC experiment showed good linear relationships at the ranges of 0.193 8-3.876 0 μg for naringin(r=0.999 0),0.109 6-2.192 0 μg for hesperidin(r=0.999 9),0.128 7-2.574 0 μg for neohesperidin(r=0.999 9),0.452 5-9.050 4 μg for baicalin(r=0.999 9).In addition,the average recoveries were 99.94%,95.83%,97.46% and 98.94%,respectively(RSD3%).CONCLUSION The method is simple,accurate and reliable with good precision.It can be used for the quality control of Dachaihu Granules.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
Access and Citation
Cited By Count: 1
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot