Title: Study on the mechanism of celecoxib inhibiting growth and inducing apoptosis of human gastric cancer cell line SGC-7901
Abstract: Objective:To study the effects of growth inhibition and apoptosis induced by celecoxib in human gastric cancer cells line SGC-7901. Methods;The gastric cell line was treated with 12. 5 - 100μM of celecoxib for 24 hours. MTT assay was used to determine the cell growth inhibition rate. Flow cytometry was used to examine cell cycle, cell apoptosis rate and MORI gene expression. Results:Celecoxib could inhibit the proliferation of gastric cells in vitro, which was associated with arresting of S phase and inducing apoptosis. The expression level of MDR1 gene decreased after treated with 25 - 100μM of celecoxib for 24 hours, but increased in 12. 5μM concentration. Conclusion:Celecoxib can induce apoptosis and block cell cycle of gastric cells. It also may reverse multi-drug resistance by descending the expression level of MDR1.
Publication Year: 2003
Publication Date: 2003-01-01
Language: en
Type: article
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