Title: Role of p38MAPK in Apoptosis of Colon Cancer Cells and Its Relationship with COX-2
Abstract: Objective To investigate the role of p38MAPK in mediating celecoxib (COX-2 selective inhibitor) inhibited the growth of tumor in colon cancer cells and its relationship COX-2.Methods The cell gowth activity of HT-29 cells after the treatment by celecoxib was observed by MTT assay, flow cytometry was used to observed the effect of celecoxib and SB203580(p38MAPK specific inhibitor ) on apoptosis and the cell cycle distribution of HT-29 cells, the expression of Phosph- p38MAPK and COX-2 protein was detected by Western blot.Results Compared with the expression of p38MAPK(0.23±0.12)and COX-2(0.95±0.14)of control group, p38MAPK expression(0.62±0.11)was higher than control group, while the expression of COX-2(0.44±0.11)was lower than control group which was treated by celecoxib. SB203580 could decrease the expression of p38MAPK(0.12±0.05)and COX-2(0.23±0.13); the expression of p38MAPK(0.43±0.12)was lower than control group, which was between celecoxib and SB203580, the decrease of COX-2 was most significant(0.15±0.10). Compared with the apoptosis of control group(4.31%), celecoxib and celecoxib+SB203580 induced apoptosis significantly(P0.01 and P0.05), the apoptosis rate of them was 40.95%、26.24% respectively.Conclusion Celecoxib can induced the apoptosis of human colon cancer HT-29 cell lines, which may through the activation of p38MAPK. In signal transduction of HT-29 cell lines, the upstream kinase of COX-2 is p38MAPK, the expression of COX-2 was regulation by p38MAPK, which has the effect of degenerative feedback regulation by COX-2. Celecoxib induced the apoptosis of tumor cells and play the role of anti-neoplasiasm through COX-2 and p38MAPK.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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