Title: Study on content determination and preparation method of iridoid glycosides in Caulis Lonicerae
Abstract:Objective To analyze loganin and sweroside contents in Caulis Lonicerae and study preparation method of loganin and sweroside.Methods A method of HPLC was developed for determination of loganin and sw...Objective To analyze loganin and sweroside contents in Caulis Lonicerae and study preparation method of loganin and sweroside.Methods A method of HPLC was developed for determination of loganin and sweroside.The content differences of the two component were analyzed,which were came from different producing area and harvesting time.The solvent extraction method and macroporous resin method were compared for enrichment of iridoid glycosides.Results HPLC method was accurate.Loganin and sweroside by the average recovery rate were 100.6%,99.8% respectively.The contents were 1.5% and 2.6% respectively.From autumn to winter,the content had a decreasing trend.Different areas had different content,the highest content were Hubei and Hu′nan.Through macroporous resin preconcentration of iridoid glycosides,transfer rates of loganin and sweroside were 47.23% and 62.35%,purity were 8.88% and 17.72%.Macroporous resin was better than solvent method.Conclusion In different producing areas and harvest time,the content of loganin and sweroside in Caulis Lonicerae have certain differences,and the harvest time of autumn is best.Macroporous resin purification of iridoid glycoside is better.Read More
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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