Title: Effect of Total Flavonoids of Bidens bipinnata on TGF-β_1/smad Pathway of Liver Tissue in Rats with Liver Fibrosis
Abstract: Objective: To study the effect of the total flavonoids of Bidens bipinnata(TFB) on the TGF-β1/ smad pathway of liver tissue in rats with liver fibrosis.Method: Liver fibrosis in rats was established by CCl4,and then the rats were randomly divided into five groups: model group,colchicine group(0.2 mg·kg-1),low-,medium-and high-dosage groups of TFB(60,120,240 mg·kg-1),as well as the normal group was set up.The drugs were given to rats daily for 30 consecutive days.The activities of alanine aminotransferase(ALT),aspartate aminotransferase(AST) and total bilirubin(TBil) in serum were examined,and the levels of hyaluronic acid(HA),laminin(LN),precollagen III(PCIII) and hydroxyproline(Hyp) in hepatic tissue were examined.The transforming growth factor-β1(TGF-β1)-immunoreactive cells were determined by immunohistochemical assay,and the expression of smad2 and smad3 in hepatic tissue was determined by Western blot.In addition,the hepatic pathological changes were observed by Masson staining.Result: Compared with model group,TFB effectively decreased the activities of AST,ALT and TBil in rats with liver fibrosis(P 0.01),the contents of HA,LN,PCIII and Hyp in homogenate were decreased(P 0.01).The quantity of TGF-β1-immunoreactive cells was reduced(P 0.01).The smad2 and smad3 protein level was down-regulated in hepatic tissue(P 0.01).Moreover,liver fibrosis was alleviated.Conclusion: TFB has protective effect on the CCl4-induced liver fibrosis in rats,the mechanism may be implicated in regulating the TGF-β1/ smad pathway to inhibit collagenation.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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