Title: Effect of Taizhi'an Capsules on the Model of Liver Fibrosis Induced by CCl_(4) in Rats
Abstract: Objective To probe into the effect of taizhi'an capsules on the model of liver fibrosis in rats. Methods 55 male SD rats were randomly divided into 3 groups: the control group(n=15),model group(n=20) and treatment group((n=20)).The model of liver fibrosis in rats of the model group and treatment group was established by subcutaneous injections of 3 mL·kg~(-1) of 40% CCl_(4) per rat twice a week for 12 consecutive weeks.After the establishment of the model,rats of the treatment group were given each 1 g·kg~(-1) of taizhi'an saline suspension(0.1 g·mL~(-1)) administered by gastrogavage q.d.for 8 consecutive weeks.Rats of the model group were given each equivalent volumes of 0.9 % sodium chloride solution via the same route as described.At the termination of the 8 week treatment course,blood samples and liver tissue were collected.Contents of malonyldialdehyde(MDA),nitric oxide(NO) and activities of nitric oxide synthase(NOS) in the serum and liver homogenate were determined.The level of collagenous fibers in the liver tissue was assayed with the Van Gieson staining.The expressions of the transforming growth factor beta 1(TGF-β1),platelet-derived growth factor(PDGF) and tissue inhibitor of metalloproteinase-1(TIMP-1) in the liver tissue were detected with immunohistochemiscal methods. Results Taizhi'an was shown to alleviate the histopathological changes of the liver and downregulate the expression of collagenous fibers and TIMP-1 in the liver tissue from rats with liver fibrosis.During the course of the liver fibrosis,the contents of MDA,NO and the expression of NOS in the serum and liver homogenate were increased and the expression of TGF-β1 and PDGF in the liver tissue was upregulated.In the present study,taizhi'an was shown to strikingly decrease the contents of MDA and NO,inhibit the expression of NOS,TGF-β1 and PDGF in the liver tissue(P0.01). Conclusion Taizhi'an was shown to exert therapeutic effects on the liver fibrosis induced by CCl_(4) in rats,probably by decreasing the contents of MDA and NO,and inhibiting the expressions of NOS,TGF-β1 and PDGF in the liver tissue.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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