Title: Effects of Huoxue Shenshi decoction on expression of Smad_4 and Smad_7 in liver of rats with liver fibrosis
Abstract: Objective:To explore the mechanism of anti-liver fibrosis effects of Huoxue Shenshi decoction(HSD).Methods:Liver fibrosis was successfully established in 50 rats by subcutaneous injection of carbon tetrachloride(CCl_4).Then model rats were divided into three groups randomly,control group,Fufang Biejia Ruangan tablet(FBRT)treatment group and HSD treatment group,and sodium chloride,FBRT and HSD were administered respectively to three groups by gavage everyday.Meanwhile,another 20 rats were arranged as normal group and were given sodium chloride by gavage everyday.After drugs intervention of 12 weeks,all rats were killed.The degree of liver fibrosis was observed through Picric Acid-Sinus Red staining of liver tissue;the expressions of Smad_4 and Smad_7 in liver tissue were determined with immunohistochemical methods;The content of HA in serum was determined with radio-immunity method.Results:The detection results of HA in serum showed that the content of HA in serum of FBRT and HSD treatment group decreased significantly compared with control group(P0.05).The classification results of liver fibrosis showed that the degrees of liver fibrosis of rats in FBRT and HSD treatment group decreased significantly compared with control group(P0.05).The detection results of immunohistochemistry showed that the expressions of Smad_4 in liver tissue in FBRT and HSD treatment group decreased obviously compared with control group(P0.05),and the expressions of Smad_7 increased obviously.Conclusion:HSD has obvious anti-liver fibrosis effect and the mechanism of this effect might be regulate expression of Smad_4 and Smad_7 in liver tissue.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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