Title: Study of anther culture in vitro of ornamental eggplant (Solanum melongena L.).
Abstract: The experiments were conducted in the induction and differentiation of anther callus and rhizogenesis of adventitious bud with ornamental eggplant (Solanum melongena L.).And then a few haploid plantlets were obtained.The results showed that the addition of 2,4-D to MS medium was essential for anther callus induction and KT also promoted callus induction.The optimal medium for anther callus induction was MS+2,4-D 0.5 mg/ L + KT 0.25 mg/ L + LH 1 000 mg/ L + Gln 500 mg/ L. The optimum medium of differentiation was MS + ZT 1.0 mg/ L +NAA 0.005 mg/ L+ GA_3 0.5 mg/ L+ LH 1 000 mg/ L. Then the adventitious buds were transferred into the rhizogenesis medium of 1/ 2MS supplemented with 0-0.005 mg/ L NAA and 20 g/ L sucrose,and rooted well. The livability was more than 90 % when aftergrowthes were transplanted.
Publication Year: 2005
Publication Date: 2005-01-07
Language: en
Type: article
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Cited By Count: 2
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