Title: Effects of Different Hormone Medium on Induction of The Callus of Chrysanthemum Leaves and Regeneration
Abstract: Explants of Chrysanthemum leaves were cultured on the media of different phytohormone combinations.Based on 6-BA and NAA in the Chrysanthemum the role of tissue culture,stated in the hormone's role in the propagation of chrysanthemum.Choose a hormone levels were proper induced callus and cluster buds.The results indicated that MS+6-BA 2.0 mg/L+NAA 1.0 mg/L medium was the best to induce callus.The MS+6-BA 2.0~3.0 mg/L+NAA 0.1~0.5 mg/L medium was the best to induce cluster buds.The higher regeneration rate was got from MS medium with 6-BA 2 mg/L+NAA 0.1 mg/L.All tube shoots can root on the 1/2MS medium with 0.1 mg/L NAA +20 g/L sucrose.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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