Title: Study on Callus Induction of Pyrethrum cinerariifolium Trev.
Abstract: The different organs of free seedlings Pyrethrum cinerariifolium seed were used as explants.Effect of different media and pH on callus induction were studied.MS as the basic medium supplemented with NAA,2,4-D,BA,KT,studies were carried out on induction of different explants of P.cinerariifolium.The results showed :MS medium was suitable for the callus induction of cotyledon leaf and hypocotyl of P.cinerariifolium,White was suitable for the callus induction of root.The best pH for the callus induction was 5.8.When cytokinin and auxin were used on callus induction,1.0 mg/L2,4-D+0.5 mg/LKT was best for cotyledon,1.0~2.0 mg/L 2,4-D +0.5 mg/L KT was best for leaves,1.0~2.0 mg/L 2,4-D+0.5 mg/L BA,0.5~1.0 mg/L 2,4-D+0.5 mg/L KT,1.0 mg/L NAA+0.5 mg/L BA,0.5 mg/L NAA +0.5 mg/L KT were best for hypocotyls,0.5 mg/L 2,4-D+0.5 mg/L BA or KT was best for roots,the inductivity could reach at 100 percent whose callus was growing,suitable for subculture.2,4-D was better than NAA,KT was better than BA for callus induction and growth of P.cinerariifolium.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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