Title: Determination of berberine hydrochloride in Shuanghuang Xiaoyan capsule by HPLC.
Abstract: Objective To establish an HPLC method for determination of the content of berberine hydrochloride in Shuanghuang Xiaoyan capsule.Methods The analysis column is Diamonsil C18(200 mm×4.6 mm,5μm),the mobile phase is Acetonitrile - 0.05 mol/L Sodium dihydrogen Phosphate - Triethylamine(26:74:0.5,and adjust pH to 4.0 with phosphoric acid),the flow velocity is 1.0 mL/min,the detection wavelength is 265 nm.External standard method is used for quantitative determination.Results Injecting sample quantity of berberine hydrochloride shows a good linear relationship with the peak area in the range between 0.02 932μg and 0.26 388μg(r =0.9 999).The average recovery rate is 95.7%,and RSD is 0.3%(n =6).Conclusions The method is simple, rapid,accurate and reliable.It is suitable for determination of the content of berberine hydrochloride in Shuanghuang Xiaoyan capsule.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot