Title: Determination of berberine in shuanghuangxiaoyan tablets by HPLC
Abstract: Aim To establish an HPLC method for determining berberine in shuanghuangxiaoyan tablets.Method C18 column(250 mm×4.6 mm,5 μm) was used and the mobile phase was composed of acetonitrile-0.05 mol·L-1KDP(25∶75).The flow rate was 1.0 ml·min-1.The detection wavelength was at 347 nm.Result The linear range of berberine was from 9.2 to 146.4 mg·L-1(r=0.999 9,n=5) and the average recovery was 99.93% with an RSD of 0.60%(n=6).Conclusion The method is accurate and simple for quality control of the preparation.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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