Title: Determination of Berberine Hydrochloride in Zhining Lotion With HPLC Method
Abstract: Objective: To establish the method for determination of berberine hydrochloride in Zhining lotion,Methods: The chromatographic condition consisited of Kromasil C18 bar(250 mm×4.6mm,5μm) with acetonitrile-0.2% orthophosphoric acid(25:75) as mobile phase at the flow rate of 1.0 ml/min,the determination wavelength was 350 nm.Results: the linear range of berberine hydrochloride was 0.4164~8.328 mg/ml,Y=6649.5X+570.29,r=0.9999,the average recovery was 97.1% with RSD% =2.98%.Conclusion: The method has high sensitivity and good reproducibility,with simple manufacture and reliable result.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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