Title: Effects of Paraquat and Maneb on System of Substantial Nigra and Striatum in Mice
Abstract: Objective To investigate the effects of paraquat (PQ),maneb (MB) or their combined exposure on neurons of substanitial nigra compact in mice,and to research the relationship between pesticides and Parkinson’s disease. Methods One hundred and twenty Kunming mice aged five weeks were randomly divided into 4 groups: control group(saline),PQ group(5 mg/kg), MB group (15 mg/kg) and PQ (5 mg/kg)+MB (15 mg/kg)group,30 in each. Mouse was treated with the pesticides through intraperitoneal injection (10 ml/kg),twice a week for 16 consecutive weeks. Spontaneous behaviors were examined by using the pole test and open field test. Histopathological examination was done and neurons were counted in substantia nigra compact. The dopamine concentration in caudate putamen (CPu) were analyzed by fluorescencespectrophotometry. Results No significant average body weight change was observed among groups and no significant difference was observed in the time of pole test and the times of crossing grids between exposed groups and control group (P0.05) before administration and after 8 weeks and 16 weeks of administration. Cell pyknosis and neurodegeneration in substantial nigra were observed in the PQ+MB group for 8 weeks and 16 weeks (P0.01). Compared with the control group,only the PQ+MB group showed increased DA concentration in CPu at the first day after 16 weeks of administration (P0.01). Conclusion Chronic exposure to low-dose PQ or MB may not induce SNc neurons damage in mice. However,chronic exposure to PQ and MB in combination can cause neurons damage and neuron loss in SNc, which clues that synergetic neurotoxic effect of PQ and MB may be involved in Parkinson’s disease.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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