Title: Comparison of subacute and chronic Parkinson disease model in mice model induced by MPTP
Abstract: Objective To compare behavioral and histopathological changes of subacute and chronic Parkinson disease (PD) mice model induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropy ridine (MPTP).Methods Subacute C57 /BL mice model was intraperitoneally injected(ip) MPTP 25 mg/kg 1 time per day for 7 days; chronic C57 /BL mice model were intraperitoneally injected (ip) MPTP 25 mg/kg 2 times per week for 5 weeks.The behavioral changes of mice was inspected by climbing pole and suspension test ; the content of DA in stratium was detected by HPLC; and the tyrosine hydroxylase (TH) expression in substantia nigra was detected by immunohistochemical staining .Re-sults Compared with that of control group ,the behavior of chronic model group was changed significantly .The suspension capability of chro-nic model group was weaker than that of subacute model group in suspension experiment .The content of DA in stratium of chronic and suba -cute model groups was decreased significantly , and in chronic group, it was significant lower than that of subacute model group (P<0.05). The amount of TH positive cells was reduced in the midbrain substantia nigra compacta .Nerve cell apoptosis was most in chronic group and relatively less in subacute group .Conclusions The chronic PD mice model induced by MPTP has more obvious and similar clinical symp -toms and is the potential stability model for further investigating PD pathology physiology mechanism .
Publication Year: 2017
Publication Date: 2017-01-01
Language: en
Type: article
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Cited By Count: 1
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