Title: DETERMINATION OF URSOLIC ACID IN PERICARPIUM GRANATI BY RP-HPLC
Abstract: [Objective] To establish a method for the determining the ursolic acid in pericarpium granati.[Methods] The content of ursolic acid in pericarpium granati was determine by RP-HPLC.A Diamonsil C18 column(4.6mm×150mm,5μm) was used and the mobile phase was methanol-aqueous solution acetic acid(pH3.4)(94 ︰ 6),the flow rate was 0.8ml·min-1,the detection wavelength was 210 nm,the column temperature was room temperature.[Results] The calibration curve was linear at the range of 10.4μg·ml-1 to 104μg·ml-1 for ursolic acid(r = 0.999 8),and the regression equation was Y = 16 315X + 55.119.The average recovery was 99.2%.[Conclusion] The method is simple,accurate and reproducible for quality control of pericarpium granati.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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