Title: HPLC determination of gallic acid in raw and processed Granati Pericarpium
Abstract: Objective:To establish a quantitative method for determination of gallic acid in Granati Pericarpium by HPLC,and compare the content of gallic acid in raw and processed Granati Pericarpium.Methods:The samples were separated at room temperature by a Diamonsil C18 column(200 mm×4.6 mm,5 μm)eluted with methanol-0.05% phosphoric acid aqueous solution(5∶95)as the mobile phase.Flow rate was set at 0.8 mL·min-1 and the detection wavelength was set at 273 nm.Results:The calibration curve was linear in the range of 0.09-0.72 μg(r=0.9997),and the average recovery(n=6)was 99.59% with RSD of 1.5%,respectively.The content of gallic acid in Granati Pericarpium processed at 180 ℃ was the highest.With the processing temperatures continue to rise,the content of gallic acid decreased.Conclusion:The method is simple,repeatable and can be used for quality control of raw and processed Granati Pericarpium.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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