Title: The effects of siRNA Inhibiting AR gene expression on proliferation and apoptosis of human blad-der cancer T24 cell line
Abstract: Objective To investigate the effects of siRNA inhibiting androgen receptor (AR) gene expression on proliferation and apoptosis of human bladder cancer T24 cell line. Methods Three small interfering RNAs targeting human AR mRNA were designed and synthesized, and then transfected in-to T24 cell line by lipofectamineTM 2000. The expression levels of AR mRNA and protein in the cells were tested by real time RT-PCR and Western blot, respectively. MTT and flow cytometry (FCM) were em-ployed to observe the proliferation and apoptosis of T24 cells after transfection. Results One siRNA suc-cessfully suppressed AR expression in T24 cells,as revealed by real time RT-PCR and Western blot analy-ses. MTT showed that siRNA could significantly inhibit the cell proliferation compared with control group (P<0.05). The apoptosis rate of T24 cells in the absence of AR was increased by 21.61%. Conclusion siRNA targeting AR mRNA can effectively silence AR gene expression,inhibit proliferation,and induce apoptosis of T24 cells significantly.
Key words:
Bladder neoplasms; Androgen receptor; RNA interfering; Apoptosis
Publication Year: 2009
Publication Date: 2009-04-08
Language: en
Type: article
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