Title: Study of the effect of siRNA targeted against survivin on cell proliferation and apoptosis of esophageal cancer cells
Abstract: Objective To investigate the effect of siRNA targeted against survivin on cell proliferation and apoptosis of Eca-109 esophageal cancer cells. Methods A siRNA plasmid expression vector against survivin was constructed and transfected into Eca-109 cells with LipofectamineTM2000.The changes of survivin mRNA and protein expression were detected by semi-quantitive RT-PCR and Western Blot.Cell proliferation was detected by MTT assay.Cell apoptosis was evaluated by flow cytometry. Results The sequence-specific siRNA effectively suppressed survivin expressing at both mRNA and protein levels.Survivin expression surpression significantly inhibited the proliferation(at 24 h and 48 h after cell seeding,the proliferation inhibition rate was 21.05% and 33.96% respectively) and induced apoptosis of Eca-109 cells(at 24 h and 48 h after transfection,apoptosis rate was(8.03±1.05)% and(6.22±1.06)% respectively. Conclusions siRNA targeted against survivin can effectively suppress Eca-109 cell proliferation and significantly induce Eca-109 cell apoptosis.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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