Title: Effects of early insulin therapy on sterol regulatory element binding protein-1c in skeletal muscle of diabetic rats
Abstract: Objective
To investigate the effect of early insulin treatment on sterol regulatory elementbinding protein-1c (SREBP-1c) in skeletal muscle of diabetic rats.
Methods
24 male Sprague-Dawley rats were randomly assigned into 4 groups of 6 animals each: normal control, untreated diabetic rats, diabetic rats treated with NPH insulin, or diabetic rats treated with gliclazide. Sterol regulatory element-binding protein 1c (SREBP-1c) messenger RNA (mRNA) expression, protein expressions of SREBP-1c, tumor necrosis factor-α (TNF-α), Tyr705 phosphorylation of signal transducer and activator of transcription 3 (p-STAT3) in skeletal muscle of diabetic rats were assayed by Western blot and real time PCR.
Results
Compared to normal rats, untreated diabetic rats had higher SREBP-1c mRNA (7.3±2.6) and nuclear protein expressions(3.3±0.4), tumor necrosis factor α (TNF-α) (0.44±0.03), and Tyr705 phosphorylation of signal transducer and activator of transcription 3 (STAT3) (0.50±0.08), which all were downregulated following insulin treatment (SREBP-1c mRNA: 4.1±1.8, SREBP-1c nuclear protein: 2.7±0.3, TNF-α: 0.19±0.22, p-STAT3: 0.29±0.03, P<0.05 for all).
Conclusion
Early insulin exerts its anti-lipotoxicity effect in skeletal muscle of diabetic rats potentially through amelioration of inhibition of lipogenesis.
Key words:
Insulin; Diabetes; Skeletal muscle; Sterol regulatory element binding protein 1c
Publication Year: 2010
Publication Date: 2010-02-27
Language: en
Type: article
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