Title: Effects of T0901317 on lipid accumulation and the expression of sterol regulatory element binding protein-1c in the liver of apoE-/-mice
Abstract: Objective To investigate the effect of T0901317 on lipid accumulation and the expression of sterol regulatory element binding protein-1c (SREBP-1c) in the liver of apoE-/- mice. Methods Male 8-week-old apoE-/- mice were randomly divided into four groups: baseline group (n = 10), vehicle group (n = 14), treatment group (n = 14) and prevention group (n = 14). All of the mice were fed a high-fat/high-cholesterol diet with or without LXR agonist T0901317 for 8 or 14 weeks. Lipid accumulation of the liver was observed by hematoxylin and eosin (HE) and oil red O staining. SREBP-1c mRNA and protein were determined by real-time quantitative PCR and western blot respectively. Results Lipid droplet of the liver in treatment and prevention group mice were markedly increased when compared with control group mice, liver structure became gradually inordinate (P 0.05). SREBP-1c mRNA and protein expression in the liver of treatment and prevention group mice up-regulated when compared with control group mice (P 0.05). Conclusion LXR agonist T0901317 can up-regulate the expression of SREBP-1c in apoE-/- mice fed with high- fat/high-cholesterol diet.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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