Title: Effects of Baicalin on barrier dysfunction of intestinal epithelial cells induced by hypoxia reoxygenation
Abstract: Objective
To investigate the effects of Baicalin on barrier dysfunction of intestinal epithelial cells induced by hypoxia reoxygenation injury.
Methods
Hypoxia reoxygenation injury models of rat's intestinal epithelial cell-6(IEC-6) were established.Optional Baicalin concentration was chosen by detecting cell proliferation by using methylthiazolyl tetrazolium(MTT) assay.IEC-6 cells were randomly divided into 5 groups: normal control group, hypoxia reoxygenation group, hypoxia reoxygenation+ 5.0×10-3 mol/L Baicalin group, hypoxia reoxygenation+ 1.0×10-3 mol/L Baicalin group, and hypoxia reoxygenation+ 0.2×10-3 mol/L Baicalin group.Supernatants were collected for the determination of malondialdehyde(MDA) and superoxide dismutase(SOD). Expressions of tight junction proteins [Occludin and Zonula Occludens 1(ZO-1)] and NF-κB protein were detected by using Western blot, and the expressions of occludin and ZO-1 mRNA were detected by using real-time fluorescence quantification-PCR.
Results
Baicalin had inhibition effects on cell proliferation induced by hypoxia reoxygenation injury(the normal control group absorbance value: 0.442±0.015, hypoxia reoxygenation group: 0.256±0.007, hypoxia reoxygenation+ 5.0×10-3 mol/L Baicalin group: 0.407±0.008; F=66.904, P<0.05). Compared with normal control group, the MDA protein level of hypoxia reoxygenation group in culture medium was increased[the normal control group: (3.502±0.231) mol/L, the hypoxia reoxygenation group: (5.541±0.143) mol/L]and SOD protein level was decreased[the normal control group: (20.073±0.493)×103 U/L, hypoxia reoxygenation group: (13.324±0.081)×103 U/L], Occludin and ZO-1 expressions were decreased, and NF-κB(p65)expression was increased(all P<0.05). Compared with the hypoxia reoxygenation group, along with Baicalin concentration increasing, MDA protein levels in the Baicalin groups were decreased[hypoxia reoxygenation+ 5.0×10-3 mol/L Baicalin group: (4.320±0.106) mol/L, hypoxia reoxygenation+ 1.0×10-3 mol/L Baicalin group: (4.578±0.080)mol/L, hypoxia reoxygenation+ 0.2×10-3 mol/L Baicalin group: (4.928±0.118)mol/L] and SOD levels were increased[hypoxia reoxygenation+ 5.0×10-3 mol/L Baicalin group: (18.462±0.284)×103 U/L, hypoxia reoxygenation+ 1.0×10-3 mol/L Baicalin group: (16.624±0.364)×103 U/L, hypoxia reoxygenation+ 0.2×10-3 mol/L Baicalin group: (14.023±0.140)×103 U/L], while protein and mRNA levels of Occludin and ZO-1 were increased, and NF-κB(P65)expression was decreased(all P<0.05).
Conclusion
Through NF-κB signaling pathways, Baicalin can alleviate intestinal epithelial cells barrier dysfunction induced by hypoxia reoxygenation.
Key words:
Baicalin; Gut barrier; Ischemia-reperfusion injury, hypoxia reoxygenation; Tight junction protein
Publication Year: 2015
Publication Date: 2015-04-05
Language: en
Type: article
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