Title: The research of that Shikonin effects on VEGF production in IL-17-stimulated HaCaT cells
Abstract: Objective To investigate whether IL-17 could stimulate the vascular endothelial growth factor (VEGF) production on HaCaT cells alone. We also investigated whether shikonin could inhibited the proinflamation effects of interleukin-17(IL-17) acting on HaCaT cells. Methods We examined the expression of VEGF by double antibody sandwich enzyme-linked immunosorbent assay ( ELISA ) and realtime polymerase chain reaction(RT-PCR) in HaCaT cells and the cell supernatant. The viability of HaCaT cells in the drug group was detected by the Cell Counting Kit-8 (CCK-8). Results The expression of VEGF in different time IL-17-stimulated groups on HaCaT cells and the cell supernatant were higher than the control group( P<0.001 ). The expression of VEGF in different drug treatment groups on HaCaT cells and the cell supematant were lower than the stimulated group by IL-17 ( P<0. 001 ). The cell viability of different drug treatment groups have no significant difference( P>0.05 ). Conclusion We show that IL-17 specifically and time-dependently augmented and induced VEGF expression on HaCaT cells and the cell supernatant Then shikonin markedly inhibited the increase tengency of IL-17 effection on HaCaT cells and the cell supematant level.
Key words:
Psoriasis; HaCaT cell ; IL-17 ; VEGF; Shikonin
Publication Year: 2011
Publication Date: 2011-08-31
Language: en
Type: article
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