Title: Human Y chromosome microdeletions: findings in 180 male infertile patients
Abstract: Objective
To investigate the characteristics of Y chromosome microdeletions among male infertility in Shenzhen, and to provide evidence for diagnosis and treatment of male infertility in clinical settings.
Methods
Included in this study were 180 patients with azoospermia or severe oligozoospermia who visited our hospital between January 2015 and January 2016. Two-tube multiplex PCR amplification and four-channel fluorescence quantification (FAM/VIC/ROX/Cy5) were used to detect microdelection at 6 sequence tag sites (STS) in three chromosomal regions (AZFa, AZFb, and AZFc).
Results
Of the 180 males with azoospermia or severe oligozoospermia, 18 (10.0%) were of AZFc deletion, and 2 (1.1%) were of AZFb+c deletion, with statistically significant difference between the two groups (P<0.05).
Conclusion
The AZFc deletion accounts for majority of Y chromosome microdeletions among male infertility in Shenzhen.
Key words:
Infertility; Y chromosome; Micronucleus, chromosomal deficit; AZF
Publication Year: 2017
Publication Date: 2017-08-15
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot