Title: Identification of 13 N-NH 3 and analysis of its radiochemical purity using high performance liquid chromatography-ultraviolet-flow-count
Abstract: Objective
To establish a new, rapid, and effective method to identify 13N-NH3 and analyze its radiochemical purity.
Methods
The chromatographic method of high performance liquid chromatography-ultraviolet (HPLC-UV) -flow-count was performed using a C18 column with mobile phase of acetonitrile and 5 mmol/L sodium 1-octanesulfonate solution (V/V=1/3) at a flow rate of 0.5 ml/min and a wavelength of 210 nm. 15% 13N-NH3 and 0.1 mg/ml NaNO3 were detected separately, and their retention times were compared and identified.
Results
The UV retention times of NH3 and NaNO3 were 2.3 and 1.4 min, respectively, and the retention times of 13N-NH3 and 13N-NOx- were 5.8 and 3.1 min, respectively. The established separation method using HPLC-UV-flow-count showed good efficiency.
Conclusion
HPLC-UV-flow-count is a simple, valid, and rapid method to identify 13N-NH3 and analyze its radiochemical purity.
Key words:
Chromatography, high pressure liquid; 13N-NH3; Radiochemical purity
Publication Year: 2015
Publication Date: 2015-05-25
Language: en
Type: article
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