Title: Determination of 5-Fluorouracil in Rabbit Serum by High Performance Liquid Chromatography
Abstract: A method for the determination of 5 _ fluorouracil in serum was proposed by an improved high performance liquid chromatography. 5 _ Fluorouracil in serum sample was extracted by acetic ether. Chromatographic separation was performed on a Diamonsil C18 column(150 mm×4.6 mm×5 μm), eluted with a mobile phase composed of methanol- water(3∶97 by volume, pH was adjected to 3.5 by glacial acetic acid) at a flow rate of 0.5 mL/min. The column effluent was monitored by UV _ absorbance detector at a wavelength of 270 nm. The chromatographic peak area was linear with mass concentration of 5 _ florouracil over the range of 0.17~42.50 mg/L. The intra _ day RSD and inter _ day RSD of the method were 0.9%~1.0%(n=5) and 1.9%~2.3%(n=5), respectively. The recovery of 92% was obtained. This assay is applicable to the determination of 5 _ fluorouracil in serum for pharmacokinetic studies.
Publication Year: 2002
Publication Date: 2002-01-01
Language: en
Type: article
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