Title: The effects of proliferation and injury in human peritoneal mesothelial cells induced by uric acid
Abstract: Objectives The study was aimed to determine the in vivo proliferation and cell injury in human peritoneal mesothelial cells (HPMC) responding to uric acid. Methods 1. Cell culture. HPMC were grown in DMEM/F12 containing 10% fetal bovine serum. When 60 -80% cells were stick on the well, they were cultured for 24 h in free serum medium so as to maintain synchronization at the phase of G0/G1. uric acid at designated concentration was added into the HPMC culture for designated periods. 2. Index. Cell proliferation which was induced by uric acid at concentrations of 200,400,600,800,1000 umol/L for 24,48,72 h respectively was measured by the CellTiter -Blue? Cell Viability assay, meanwhile, the levels of LDH in cultivating medium at different times for different concentrations was detected by LDH Kit. Results To the control group, incubation with 200 -1000 umol/L uric acid inhibited cell proliferation, which was decrease gradually with the increase of time and uric acid concentration rises(p < 0.05). Incubation with various concentrations (200 ~ 1000 umol/L) uric acid increased the level of LDH at dose -dependent and time -dependent manner in HPMC. Conclusions The results indicate that uric acid may inhibit proliferation of HPMC and led to HPMC damage in vitro.
Key words:
Uric Acid; Pertoneum; Stromal Cells Cell Division
Publication Year: 2009
Publication Date: 2009-09-15
Language: en
Type: article
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