Title: The effect of insulin like growth factor 1 on the cytotoxicity of high concentration glucose to human peritoneal mesothelial cells
Abstract: Objective: To compare the effect of insulin like growth factor 1(IGF 1) and epidermal growth factor (EGF) on the cytotoxicity of high concentration glucose to human peritoneal mesothelial cells (HPMCs). Methods: Human peritoneal mesothelial cells were cultured in vitro , and different concentration of IGF 1 or EGF was added to HPMCs that was cultured in RPMI 1640 medium containing 83.3 mmol·L -1 glucose. After 24, 48 and 72 hours, the incorporation of 3H thymidine ( 3H TdR) and the concentration of lactate dehydrogenase(LDH) in supernatants were detected. Results: The addition of 50 μg·L -1 of IGF 1 to 83.3 mmol·L -1 glucose caused a (75±15)% increase in 3H TdR incorporation for 72 hours ( P 0.01), and a (34±8)% decrease in release of LDH for 24 hours ( P 0.01). EGF had no significant influence on the cytotoxicity of high glucose to HPMCs. Conclusion: IGF 1 can reverse the cytotoxicity of high glucose to HPMCs.
Publication Year: 1999
Publication Date: 1999-01-01
Language: en
Type: article
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