Title: Effect of Smad3 siRNA on transforming growth factor-β-induced MMP-9 expression in MDA-MB-231 cells
Abstract: Objective To investigate whether Smad3 small interference RNA (siRNA) can in-hibit transforming growth factor (TGF)-β-induced MMP-9 gene expression in MDA-MB-231 cells. Meth-ods siRNA targeting Sroad3 was designed, synthesized, then transfected into MDA-MB-231 cells and stimulated by TGF-β1 (1 μg/L). After 24 h of stimulation,Smad binding element (SBE) reporter activi-ties were measured by a luciferase reporter assay. After 24 h of stimulation, Smad3 protein expression was detected by immunoblot analysis. After 48 h of stimulation, MMP-9 mRNA and protein expression was de-termined by RT-PCR and by immunoblot analysis respectively. Results Smad3 siRNA inhibited the Smad3 protein expression (0.5 ± 0. 1 vs 1.0 ± 0. 2). Smad3 siRNA suppressed TGF-β-induced 4 × SBE luciferase reporter activity (5.2 ± 0.3 vs 1.0 ± 0. 1 ;P < 0. 05). Compared with the control, Smad3 siRNA suppressed TGF-β1-induced MMP-9 mRNA and protein expression (1.3 ± 0.2 vs 2.4 ± 0. 3 ;1.1 ± 0.2 vs 1. 8 ± 0.4). Conclusion Smad3 siRNA can effectively down-regulate TGF-β-induced MMP-9 gene ex-pression in MDA-MB-231 cells,which could provide a novel approach for therapeutic intervention to treat breast cancer.
Key words:
Breast carcinoma; siRNA; TGF-β ; Smad3 ; MMP-9
Publication Year: 2009
Publication Date: 2009-10-08
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot