Title: Effect of transfecting anti-TGF-β_1 siRNA on TGF-β/Smad signal transmission pathway in the liver cells by different hepatic targeting methods
Abstract: Objective To compare the difference of effect on the anti-hepatic injury and fibrosis and the influence of TGF-β/Smad signal transmission pathway with four transfection methods by anti-TGF-β1 siRNA entered target cells.Methods The 50 rats priming TGF-β/Smad signal transmission pathway in the liver tissues were randomly divided into 5 groups: the model control group(group M,n=10),the vena caudalis straight injection group(group V,n=10),the liposome package group(group L,n=10),the hydrodynamics group(group H,n=10) and hepatic artery catheterization group(group A,n=10).TGF-β1 siRNA plasmids were introduced into target cells in the liver by different methods. Serum ALT,AST,HA were detected to evaluate the effect of four transfection methods;liver tissue was detected by HE staining;expression of type Ⅰ and Ⅲ collagen,TGF-β1 and Smad3 mRNA were detected by RT-PCR;expression of type Ⅰ and Ⅲ collagen,TGF-β1 and Smad3 protein were detected by immunohistochemistry technology.Results The anti-TGF-β1 siRNA plasmids transfected into target cells by each method effectively blocked the TGF-β/Smad signal transmission pathway in the rats' hepatic tissues(P0.05).The effect in group L and group A was significantly better than that in the group V and group H(P0.05),there was no significant difference between group A and group L(P0.05).The effect in the group V was the worst (P0.05).Conclusion The anti-TGF-β1 siRNA plasmid transfecting by hepatic artery catheterization is a safe and high-performance method for hepatic targeting transfection of foreign gene.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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