Title: Preparation and characterization of Smad2 specific siRNA in human keloid fibroblast
Abstract: Objective:To study the inhibition effect of Smad2 specific small interfering RNA(siRNA) on Smad2 gene expression in human keloid fibroblast,searching for the most efficient Smad2 specific siRNA.Methods: According to the principle of siRNA design,Smad2 specific siRNA(1-3) was designed and the keloid fibroblast were transfected.The level of Smad2 expressions were detected using semi-quantity RT-PCR. Results: Endogenous Smad2 expression were efficiently blocked in keloid fibroblast by Smad2 siRNA-3 in a dose- and time-dependent manner.Smad2 expression decreased significantly along with the increase of siRNA-3 dose within 50-200 nmol/L and was the lowest 24 h after transfection with 200 nmol/L siRNA-3. Conclusion: The expression of Smad2 in keloid fibroblast can be blocked efficiently in a dose- and time-dependent manner by specific siRNA.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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