Title: Expression of checkpoint kinase 1 in esophageal squamous cell carcinoma and its significance
Abstract: Objective To investigate the expression of checkpoint kinase 1 (Chk1) in esophageal squamous cell carcinoma (ESCC) and its significance.Methods hnmunohistochemistry and in situ hybridization methods were used to detect the expression of Chk1 gene in 62 cases of ESCC,31 cases of adjacent dysplasia and 62 cases of normal esophageal mucosa.Results The expression of Chkl mRNA and protein was gradually increased from normal esophagus mucous tissue (protein:14.5%,mRNA:82.3%),adjacent atypical hyperplasia tissue (protein:38.7%,mRNA:35.5%) to ESCC tissue (pro-tein:88.7%,mRNA:8.1%),and there was significant difference among three groups (P < 0.05).The expression levels of Chk1 mRNA and protein in the deep layer invasive groups (protein:94.5%,mRNA:85.5%) were considerably higher than those in infiltrating shallow layer group (protein:42.9%,mRNA:57.1%),and there was significant difference among the groups (P < 0.05).The expression levels of Chk1 mRNA and protein in lymphatic metastasis groups (protein:100.0%,mRNA:95.0%) were significantly higher than those in non-lymphatic metastasis group (protein:83.3%,mRNA:76.2%),and there was significant difference among the groups (P < 0.05).The expression levels of Chk1 mRNA and protein in poor differentiated ESCC tissues were markedly higher than those in well differentiated tissues (P <0.05).Conclusion The expression of Chk1 protein and mRNA was closely associated with the invasion and metastasis of ESCC.
Key words:
Esophageal squamous carcinoma; Checkpoint kinases 1 ; Infiltration; Metastasis
Publication Year: 2014
Publication Date: 2014-05-08
Language: en
Type: article
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