Title: An experimental study of the effects of Naloxone on IL-12P40 in Parkinson disease
Abstract: Objective To evaluate the effects of Naloxone on cytokine interleukin-12 P40(IL-12P40) expression in animal models of Parkinson disease(PD).Methods 30 SD rats were randomly divided into 3 groups,10 in each group.The first group(PD group) of ten rats were prepared to be hemiparkinsonian models induced by unilateral stereotaxic infusion of 6-hydroxydopamine(6-OHDA) in right substantia nigra of mid-brain.Every week after operation,the circles the rats run were observed in 30 minute after intraperitoneal injection apomorphine.If the rats ran more than 6 circles every minute at the fourth week after operation, they were successful PD rat models.The second group(Naloxone group) were given stereotactic injection of naloxone in rat substantia nigra of the right side,and then the PD models were produced by the same way as the first group.The third group injected with normal saline as a control group.From the operative side in rat substantia nigra,an immunohistochemistry(IH) technique was used to detect the expression of IL-12P40.Results IH measurement showed,compared to control group,IL-12P40 in PD group elevated significantly(P0.01),and compared to PD group,the expression of IL-12P40 in Naloxone group was significantly less(P0.01).Conclusion Naloxone is effective on reducing incidence of PD,one of its important intervention mechanism may be influencing the expression of IL-12P40.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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