Title: Antagonism of tert-butylhydroquinone to sodium arsenite-induced cytotoxicity in Chang liver cells in vitro.
Abstract: Objective To study the antagonism of tert-butylhydroquinone(tBHQ) to NaAsO2 induced cytotoxicity in Chang liver cells in vitro.Methods Chang liver cells were exposed to NaAsO2(0,20,40,60 and 80 μmol/L) for 24 and 48 hours,or Chang liver cells were treated with tBHQ(5 and 20 μmol/L),then exposed to NaAsO2(40 and 60 μmol/L) for 24 and 48 hours.The conditions of control group was the same as NaAsO2 group.Alamar Blue was used to evaluate the viability of cells.Results Chang liver cells were exposed to NaAsO2 for 24 and 48 hours,Alamar Blue reduction rates decreased significantly and Alamar Blue reduction rates of 48 hours group were lower than 24 hours group(P 0.01).Alamar Blue reduction rate of 5 μmol/L tBHQ pretreatment group was higher than 60 μmol/L NaAsO2 group for 24 h(P0.01);Alamar Blue reduction rate of 20 μmol/L tBHQ pretreatment group were higher than respective NaAsO2 group for 24 h(P0.05).Alamar Blue reduction rate of 5 μmol/L tBHQ pretreatment group were higher than respective NaAsO2 group for 48 h(P0.01);Alamar Blue reduction rate of 20 μmol/L tBHQ pretreatment group was higher than 40 μmol/L NaAsO2 group for 48 h(P0.01).Conclusion tBHQ can decrease the cytotoxicity induced by NaAsO2 in Chang liver cells,increase the resistance to the cytotoxicity induced by NaAsO2 in Chang liver cells.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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