Title: Effects of sodium arsenite on the reactive oxygen species levels and cell apoptosis of human normal liver cells
Abstract: Objective
To investigate the effects of sodium arsenite (NaAsO2) on cell survival circumstance, reactive oxygen species (ROS) and cell apoptosis in human normal hepatic cells (L-02).
Methods
L-02 cells were exposed to different doses of NaAsO2 (0, 50, 100, 150 μmol/L) for 24 h. MTT assay was used to detect the survival of L-02 cells, and flow cytometry (FCM) was used to detect the ROS levels and the early (Q4), late (Q2) apoptosis of L-02 cells.
Results
Cell survival rate: cell survival rate was compared between groups, the difference was statistically significant (F= 350.51, P < 0.05), the cell survival rates of 50, 100 and 150 μmol/L NaAsO2 groups [(87.30 ± 3.74)%, (49.03 ± 4.72)%, (13.44 ± 4.01)%] were significantly lower than that of the control group [(100.00 ± 0.00)%, all P < 0.05]; compared with 50 μmol/L NaAsO2 group, the cell survival rates of 100 and 150 μmol/L NaAsO2 groups were significantly decreased (all P < 0.05); compared with 100 μmol/L NaAsO2 group, the cell survival rate of 150 μmol/L NaAsO2 group was significantly decreased (P < 0.05). The ROS levels: ROS levels were compared between groups, the difference was statistically significant (F= 407.78, P < 0.05), the ROS levels of 100 and 150 μmol/L NaAsO2 groups (3 212.00 ± 221.93, 5 521.33 ± 179.63) were significantly higher than that of the control group (1 691.67 ± 73.98, all P < 0.05); compared with 50 μmol/L NaAsO2 group (1 927.67 ± 62.45), the ROS levels of 100 and 150 μmol/L NaAsO2 groups were significantly increased (all P < 0.05); compared with 100 μmol/L NaAsO2 group, the ROS level of 150 μmol/L NaAsO2 group was significantly increased (P < 0.05). Cell apoptosis: cell apoptosis rates of Q2, Q4 and Q2+ Q4 were compared between groups, the differences were statistically significant (F= 256.84, 26.53, 63.89, all P < 0.05); excecpt the cell apoptosis rate of Q4 in 50 μmol/L NaAsO2 group [(5.43 ± 0.57)%], the cell apoptosis rates of Q2 [(5.67 ± 0.21)%] and Q2+ Q4 [(11.10 ± 0.40)%] in 50 μmol/L NaAsO2 group, the cell apoptosis rates of Q2 [(13.60 ± 0.79)%], Q4 [(7.37 ± 2.01)%] and Q2+ Q4 [(20.97 ± 2.38)%] in 100 μmol/L NaAsO2 group, the cell apoptosis rate of Q2 [(13.47 ± 0.78)%], Q4 [(16.97 ± 3.45)%] and Q2+ Q4 [(30.43 ± 3.84)%] in 150 μmol/L NaAsO2 group were significantly higher than those of the control group [Q2: (3.47 ± 0.12)%, Q4: (2.90 ± 0.90)%, Q2+ Q4: (6.37 ± 1.00)%, all P < 0.05]; compared with 50 μmol/L NaAsO2 group, the cell apoptosis rates of Q2, Q4 and Q2+ Q4 in 100 and 150 μmol/L NaAsO2 groups were increased, except the cell apoptosis rate of Q4 in 100 μmol/L NaAsO2 group, the differences were statistically significant (all P < 0.05); the cell apoptosis rates of Q4 and Q2+ Q4 in 150 μmol/L NaAsO2 group compared with 100 μmol/L NaAsO2 group were significantly increased (all P < 0.05).
Conclusions
NaAsO2 can induce L-02 cells to increase ROS levels, and inhibit L-02 cell proliferation. In addition, NaAsO2 can induce early apoptosis and late apoptosis in L-02 cells.
Key words:
Arsenites; L-02 cells; Reactive oxygen species; Cell apoptosis
Publication Year: 2017
Publication Date: 2017-01-20
Language: en
Type: article
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