Title: Mechanisms of primary-cultured hepatocyte apoptosis induced by dimethoate in rats.
Abstract: To study the effects of dimethoate on liver cell apoptosis,dimethoate was added to hepatocyte culture fluid of rats with final concentration of 0,3,10,30,100 and 300 μmol/L,respectively.After 12 h and 24 h exposure,apoptotic cells were observed by electron microscope and fluorescence microscope,apoptosis rate was analyzed by annexin V/PI method;intracellular Ca2+ concentration,reactive oxygen species(ROS) and mitochondria membrane potential(Δψm) changes were analyzed by Fluo-2/AM,dichlorofluorescin diacetate(DCFH-DA) and rhodamine 123 method respectively.The results showed that after 12 h and 24 h exposure,ultrastructural changes of apoptotic cells induced by dimethoate were observed,cell apoptosis rate increased significantly,experimental groups(apart from the group with 3 μmol/L) had significant(P0.05 or P0.01) differences with control group and followed dose proportionality.Intracellular Ca2+ concentration was significantly(P0.01) high in group with 3 μmol/L was than that of control group,and then decreased with increasing dosage.Intracellular ROS level increased with increasing dosage and time of exposure in the range of 3-100 μmol/L;however,ROS level decreased slightly in group with 300 μmol/L.Not concluding group with 3 μmol/L,the differences between control group and experimental groups were significant(P0.01).Δψm decreased continuously except group with 300 μmol/L after 24 h exposure.It was concluded that low dosage of Dimethoate can induce liver cell apoptosis,and intracellular Ca2+,ROS and Δψm may participate this process.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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