Title: Rat hepatocyte lipogenesis and insulin-stimulated lipogenesis: comparison of metabolite effects and methods of measurement.
Abstract: This study compared the effects of certain metabolites (either singly or in various combinations) and the methods of measuring lipogenesis (using either 14C-acetate or 3H2O incorporation into lipids) on total lipid synthesis and insulin-stimulated total lipid synthesis in the isolated rat hepatocyte. There were quantitative and qualitative differences between 14C-acetate and 3H2O incorporation into lipids; metabolites acutely affected both lipogenesis and insulin-stimulated lipogenesis with either isotope; and insulin's effect on lipogenesis was greater when measured by 14C-acetate incorporation. It is suggested that a particular choice of incubation media and isotope may inadvertently bias a study of insulin-stimulated lipogenesis and that metabolite supply plays a major role in regulating insulin-stimulated lipogenesis.
Publication Year: 1987
Publication Date: 1987-04-01
Language: en
Type: article
Indexed In: ['pubmed']
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Cited By Count: 2
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