Title: Study on isolation and identification of Aspergillus oryzae from bovine rumen and analysis of its effect on corn straw degradation
Abstract: In order to increase cellulose degradation of corn straw by microorganisms,the aim of this study was to isolate cellulose-degrading microorganism from bovine rumen and analyse its effect on straw utilization.The microorganism was first screened in selective culture medium for cellulase detection,and then cultured in corn straw medium.One superior cellulose decomposing strain(No.3) was isolated from bovine rumen and identified as Aspergillus oryzae by morphological and 26S rDNA technique,which was used for solid fermentation of corn straw to determine cellulase characteristics and fermentation results.The results showed that the optimum pH and temperature of cellulase were 5.5~6.0 and 50 ℃;the CMCase and filter paper cellulase activity were 6.01,1.15 U·g-1,respectively.Compared with the control,the contents of glucose and soluble protein in the corn straw fermented with A.oryzae were increased by 123.53%,48.98%(P0.05),and the degradation rates of cellulose and hemi-cellulose were 25.01%,45.99%,respectively(P0.05).The results indicated that A.oryzae had high application value,and it could be used as a potential strain for straw utilization.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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Cited By Count: 1
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