Title: Study on the Optimization of Culture Conditions for Promoting the Crude Protein Content of Fermentation Corn Straw Substrate and Activity of Relevant Enzymes under the Solid-state Fermentation with Mixed Strain
Abstract: [Objective] The aim was to study the optimization of culture conditions for promoting the crude protein content of fermentation corn straw substrate under the solid-state fermentation with mixed strains and discuss the relationship between crude protein content and the activity of relevant enzyme systems of the fermented corn straw substrate.[Method] Different kinds of yeasts(Saccharomyces cerevisiae,Candida tropicalis and Candida utilis),inoculation volume of yeast(5%,10% and 20%) and inoculation time were used as factors to make orthogonal experiment.The optimization of culture conditions for promoting the crude protein content of fermentation corn straw substrate under the solid-state fermentation with Pleurotus ostreatus and yeasts were studied.Meanwhile,the activities of ligninolytic enzymes,filter paper enzyme and carboxymethyl cellulose under different culture conditions were studied.The relationship between the between crude protein content and the activity of relevant enzyme systems of the fermented corn straw substrate was discussed.[Result] The results of the orthogonal experiment showed that the crude protein content of fermentation substrate was highest(13.36%)under A3B2C1 combination culture conditions.Under these fermentation conditions,the activities of laccase and manganese peroxidase of the fermentation substrate were also highest during the 5th-15th day.But activities of filter paper enzyme and carboxymethyl cellulose under different combination culture conditions had no obvious difference.[Conclusion] The optimal culture conditions for promoting the crude protein content of fermentation corn straw substrate under the solid-state fermentation was as follows:inoculating 10% liquid strain producing C.utilist at the same time of inoculating P.ostreatus.The activity of ligninolytic enzyme system might have a positive correlation with crude protein content of the fermented corn straw substrate.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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