Title: Phenotypic Changes of Renal Tubulointerstitial Cells in Rats Glomerulosclerosis and Effect of Shenshuning Mixture
Abstract: Objective:To explore whether there is phenotypic changes of renal tubulointerstitial cells in glomerulosclerosis rats and study the effect of Shenshuning mixture on it.Methods:Sprague-Dawley rats were randomly divided into 4 groups, sham operation group, glomerulosclerosis model group, glomerulosclerosis treated with Shenshuning group and glomerulosclerosis treated with benazepril group. Glomerulosclerosis model rats were induced by unilateral nephrectomy(Nx)and Doxorubicin injection. At the end of 8 weeks, Morphological expression and ultrastructure changes were observed by lightscope and electronlicroscopy. Immunohistochemical stain to explore the expression of α-smooth muscle actin(α-SMA)and transforming growth factor-β 1(TGF-β 1) in tubulointerstitial cells, and the relative positive area of α-SMA, TGF-β 1,and interstitial fibrosis area were analyzed using compute-assisted image analysis software, while examined weight, 24 hour proteinuria,plasma albumin, total serum proteim and renal function.Results:There was littleα-SMA expression,trace TGF-β 1 positive was found in the sham operation groups'tubulointerestitial, but prominent α-SMA and TGF-β 1 positive staining in tubulointersititial cells of model group(P0.01), intersititialfibrosis and abundance lymphocyte and monocyte soakage, marked with deduce of albumin, total serum proteim(P0.01)and increase of 24 hours proteinuria excretion, blood urine nitrogen and creatinine(P0.01).The 24 hours proteinuria excretion,blood urea nitrogen and creatinine was remarkably reduced after treated with Shenshuning mixture and benazepril(P0.01), also with the lessened of the α-SMA positive area, TGF-β 1 area and interstitial area(P0.01). The relatively analysis show significant corelation of α-SMA and TGF-β 1 factors(r=0.637,P0.01). Conclusion:The expression of α-SMA in tubulointerstitial cells in glomerulosclerosis rats is pronounced, indicate that phenotypic changes of tubulointerstitial cells involvement in the pathogenesis of glomerulosclerosis nephropathy. Shenshuning and Benazepril can inhibit the phenotypic changes of tubulointerstitial cells and TGF-β 1 expression,impair interstitalfibrosis. Shenshuning mixture was better than benazeril at reducing 24 hour proteinuria, but no more significance (P0.05). Shenshuning mixture is efficient strategy for treating glomerulosclerosis.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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