Title: Experimental Study on Effect of Shenluoning (SHLN) on IgAN Model Rat Tubulointerstitial Lesions
Abstract: Objective:To explore the tubulointerstitial lesions of experimental IgA nephrosis (IgAN) model rat, and to observe the curative effect of compound Shenluoning (SHLN) on it.Methods:Sprague- Dawley rats were randomly divided into 4 groups: blank group, IgAN model group, SHLN group and Shenyankangfupian (SYKFP) group. The IgAN rat model was established by injecting steophylococcus enterotoxin B into rat caudal vein, feeding bovine serum albumin. Rats were sacrificed at week 16 to detect blood unine nitrogen (BUN), serum creatinine (Scr), urine crythrocyte, 24 hours urine protein excretion (UPE) and urine D-N-Acetyl glucosaminidase (NAG). The renal tissues were studied by light microscopy, electron microscopy and immunofluorescent examination. In situ hybridization(ISH), immunohistochemistry and computer image analysis were used to investigate the pathological change of tubulointerstitial lesions, and to measure the expression of collagen Ⅲ (Col-Ⅲ)、transforming growth factor-β_1 (TGF-β_1), plasminogen activator inhibitor-1(PAI-l), matrix metalloproteinase(MMP-1),tissue inhibitor of metalloproteinase-1(TIMP-1).Results:(1) Model group: Degeneration in renal tubular epithelial cells necrosis; mitochondrion structure disappear and quantity decrease; renal Interstitial fibroblasts proliferation and collagen fiber deposition; the content of urine erythrocyte, urine protein, BUN, SCr and NAG elevated significantly; the expression of Col-Ⅲ、TGF-β_1、PAI-1、TIMP-1 increased remarkably; the expression of MMP-1 reduced.(2) SHLN group and SHYKFP group: The rats’ tubulointerstitial lesions markedly decreased; content of urine erythrocyte, urine protein, BUN, Scr and NAG are much lower than those of model group; the expression of Col-Ⅲ、TGF-β_1、PAI-1、TIMP-1decreased evidently; the expression of MMP-1 increased remarkably.Conclusion:SHLN can observably alleviate tubulointerstitial pathologic injury of IgAN model rats; ameliorate renal function; stayed progression of renal interstitial fibrosis (RIF) and renal failure.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot