Title: Study on dynamic change of carotenoids contents in tomato by HPCE.
Abstract: A high performance capillary electrophoresis(HPCE)method was found for measuring the content of lycopene andβ-carotene in tomato.The samples were extracted with the mixed solvent of acetone and petroleum ether by ultrasonic treatment,which were used as the test solution after separation and purification.The optimal experimental conditions,such as concentration and pH of buffer,separation voltage,separation temperature and injection time on the separation efficiency were determined first.The experimental conditions were as the followings:detection wave length was 450nm,running buffer was pH 4.5,HAc-NaAc was 20mmol/L,electrophoresis voltage was 24kV,injection time was 6swith the pressure of 0.5 MPa,electrophoresis temperature was 20 ℃.The samples could be completely separated in 16 min with a good linear relationship under the above conditions.The proposed method could be simultaneously applied for the determination of lycopene andβ-Carotene in tomato plant samples with the advantages of high accuracy,fast separation and simple extraction.Furthermore,the contents of tomato sample with five different ripening periods were measured and analyzed in accordance with the optimal conditions of HPCE.The results indicated that the content ofβ-carotene was decreased firstly and then gradually increased with the content of lycopene increased during the different mature phases,and both of the contents would peak at the completion of their ripened phases.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot