Title: Clinical application of PCR-SSCP in detecting Mycobacterium tuberculosis drug resistance
Abstract:AIM To study the relationship of gene mutation and drug resistance in Mycobacterium tuberculosis and to evaluate the clinic application of PCR SSCP. METHODS: katG, rpoB, rpsL genes mutation in 58 clin...AIM To study the relationship of gene mutation and drug resistance in Mycobacterium tuberculosis and to evaluate the clinic application of PCR SSCP. METHODS: katG, rpoB, rpsL genes mutation in 58 clinical isolated strains of Mycobacterium tuberculosis were detecting by PCR SSCP and traditional drug susceptibility test, and the results were compared. RESULTS: The results of drug susceptibility test showed that there were 41 multi drug resistant strains in the 58 clinical isolated organisms. The number of three drug resistant strains was 21 (51.2%) and that of two drug resistant strains was 14 (34.1%), but only 6 (14.6%) strains were one drug resistant. The gene mutation rate of INH, RFP and SM was 40% (23/58), 45% (26/58) and 38% (22/58) by PCR SSCP in the 58 clinical isolated strains and the rate of one, two and three gene mutations was 32%, 29% and 23.8%, respectively. The coincidence rate of three drug resistance in isolates by susceptibility test and PCR SSCP was 61.9% (13/21), and those of two drug and of one drug were 85.7% (12/14) and 50% (3/6). The mutation rate of high resistant strains was 80.5% (62/77) and low resistant strains 60% (12/20). CONCLUSION: The detecting rate of two drug resistant strains and three drug resistant strains was higher and the mutation rate increases with the level of drug resistance. The combined application of PCR SSCP and traditional drug susceptibility test can enhance the detecting rate.Read More
Publication Year: 2003
Publication Date: 2003-01-01
Language: en
Type: article
Access and Citation
Cited By Count: 1
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot