Title: Application of PCR-SSCP Technique to Detection of Drug Tolerant Genetic Mutation in M.Tuberculosis among Coal Worker's Pneumoconiosis Patients Complicated with Tuberculosis
Abstract:Objective To study the relationship of drug tolerant genetic mutation and drug tolerance in M.tuberculosis and to explore the value of clinical application of PCR-SSCP.Methods A total of 32 clinical i...Objective To study the relationship of drug tolerant genetic mutation and drug tolerance in M.tuberculosis and to explore the value of clinical application of PCR-SSCP.Methods A total of 32 clinical isolated strains of M.tuberculosis were collected among 102 coal worker's pneumoconiosis patients complicated with tuberculosis.The genetic mutation of katG,rpoB and rpsL were detected by PCR-SSCP,and the results were compared with those by traditional antimicrobial susceptibility test(AST).Results The genetic mutation rates of katG,rpoB and rpsL by PCR-SSCP were 43.75%(14/32),53.13%(17/32)and 31.25%(10/32)respectively.The tolerance results of INH,RFP and SM by AST were 53.13%(17/32),65.63%(21/32)and 40.63%(13/32).Results showed that there were 19(59.38%)multi-drug tolerant strains in 32 clinical isolated strains,including 11(42.31%)three-drug tolerant strains,8(30.77%)two-drug tolerant strains,and 7(26.92%)one drug tolerant strains.The rate of total drug tolerance was 81.25%.By PCR-SSCP,7 strains of allied mutation of three genes,6 of two mutations and 8 of only one were detected.The compliance rates with AST method were 63.64%(7/11),50.00%(4/8)and 57.14%(4/7)respectively.Conclusions PCR-SSCP technique is applicable to the screen of drug tolerant genetic mutation of katG,rpoB and rpsL in M.tuberculosis among the pneumoconiosis patients complicated with tuberculosis.When the technique is applied in combination with AST method,it would be of great significance in the clinical prescription.Read More
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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