Title: Preparation and recognition properties of tyramine molecularly imprinted polymers
Abstract: A polymer used for rapid determination of tyramine was prepared by molecular imprinting technology.The uniformly imprinted polymer microspheres were prepared by precipitation polymerization in acetonitrile using tyramine as template,methacrylic acid(MAA) as functional monomer,azobisisobutyronitrile(AIBN) as initiator and ethylene glycol dimethaerylate(EGDMA) as cross-linker,respectively.The UV spectrophotometry was used to demonstrate the interaction between tyramine and MAA.It was found that in this study one tyramine was entrapped by one MAA molecules,which was the main existing form of subject and object.The absorption kinetics of imprinted polymer were studied,as well as the adsorption efficiency by equilibrium adsorption experiments.The results showed that the imprinted polymer microspheres reached equilibrium gradually after 8 h and had higher adsorption efficiency than the non-imprinted polymer microspheres,which had the same chemical composition.The recognition properties were analyzed by Langmuir model.The Scatchard chart showed the apparent maximum binding capacity(Bmax) and dissociation content(KD) of the imprinted polymer were 325.0μmol/g and 0.577mmol/L,respectively.The absorption selectivity of imprinted polymer was nice.The tyramine imprinted polymer microspheres synthesized in this method had good performance and could be used for separation,enrichment and analysis of tyramine in foods.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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