Title: Simultaneous Quantitative Determination of Viterxin,Quercetin and Quercitrin in Polygonum viviparum in Tibet Plateau by RP-HPLC
Abstract: To develop a sensitive and specific reversed-phase high performance liquid chromatography (RP-HPLC) method for simultaneous determination of three flavoniods in Polygonum viviparum,the HPLC separation was performed on a Kromasil C_18 column(250 mm×4.6 mm,5 μm) and detected with DAD at 360 nm. The gradient elution was carried out with methano-H_2O(containing 0.25% phosphoric acid) as the mobile phase at a flow rate of 1 mL/min.The results indicated that the calibration curves for viterxin,quercetin and quercitrin were linear over the ranges of 8-40 μg/mL,5-50 μg/mL and 5.33-52 μg/mL,their assay recoveries were 100.3%, 98.9% and 100.4%,their intraday repeatability were 0.78%,0.51% and 0.38%,and interday repeatability were 2.6%,1.9% and 2.1%,respectively.This method was simple,accurate and sensitive,so it could be used for the quality control of P.viviparum.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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Cited By Count: 4
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