Title: The effect of berberine on the expression of glucose transporter 1 in the kidney of type 2 diabetes mellitus rats
Abstract:Objectives To investigate the effect of berberine on the expression of GLUT1 in the kidney of type 2 diabetes mellitus rats,and the mechanism of the protective effect of berberine on kidney,which can ...Objectives To investigate the effect of berberine on the expression of GLUT1 in the kidney of type 2 diabetes mellitus rats,and the mechanism of the protective effect of berberine on kidney,which can be useful for the clinical application to prevent and cure diabetic nephropathy.Methods 45 healthy male SD rats were adaptively fed for one week,and then ten were randomly selected from them as the normal control group,which were given ordinary feed.The remaining rats were fed with high-lipoglucose food for two monthes and injected STZ through caudal vein to establish the type 2 diabetes mellitus model.The model rats,were randomly divided into 2 groups:diabetic model group(DM group,13) and berberine treatment group(BBR group,13).After approciate treatment for 8 weeks,each group's BW,KW/BW,FBG,TC,TG,Scr and BUN were measured,and the expression of GLUT1 was qualitatively and hemi-quantitatively detected by the methods of immune Western blot and immunohistochemistry.Results The general condition and the changes of biochemical indicators of the BBR group were superior to those of the DM group;compared with the normal control group,the GLUT1 expression of the DM group remarkably increased(P0.01);compared with the the DM group,the GLUT1 expression of the BBR group strikingly decreased(P0.01).Conclusions Berberine can effectively decrease blood sugar and blood fat.In addition,Berberine can also distinctly protect the kidneys of the rats of the type 2 diabetes mellitus,the mechanism of which are realted to decreasing the expression of GLUT1 and TGF-β1,regualting the generation and degradation of extracellular matrix,and putting off the process of kidney fibrosis.Read More
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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